Room 64 is one of the rooms of the Hotel Cortez.
After Gabriel checks in, he is settled in Room 64 and is raped by the Addiction Demon. When Gabriel dies, John Lowe comes to check out the room after receiving a mysterious anonymous call. He rents the room and starts temporarily living in the room when Alex decides to push him out of the family home. He also has sex with Agnetha and Vendela's ghosts in the room after they seduce him while looking for their purpose after death. Afterwards, John checks out.
The Hidden Chamber
Behind a wardrobe in the room there is a hidden chamber. It's used to keep "memories" of every murder commited following The Ten Commandments. Two of the victims were killed by James March and the rest by John Lowe. The trophies include a thief's hand, field workers' teeth, a man's tongue and eyes, twin brothers' hearts, Martin Gamboa's brain, gossip website editors' vocal cords, a television priest's spleen, Detective Hahn's testicles, and The Countess' head. They are all stored in glass jars with golden labels of the commandment that they had broken.